tov 21g Search Results


96
ATCC tov21g cells
Tov21g Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tov+21g/us12595263-677-0-8?v=ATCC
Average 96 stars, based on 1 article reviews
tov21g cells - by Bioz Stars, 2026-07
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93
ATCC human ovarian adenocarcinoma
Expression of murine mesothelin. a RT-PCR detection of mesothelin (M, 449 base pairs) and control GAPDH (G, 473 bp) RNA in Panc02 cells. b Mesothelin protein surface expression – cells were stained with rat anti-mouse mesothelin monoclonal antibody B35 ( black line ) or normal rat IgG ( gray line ) and Cy5-conjugated donkey anti-rat IgG and analyzed by flow cytometry. Top right panels show that Panc02 surface detection is reduced by trypsin treatment compared to scraping of the adherent cells into solution. Adherent Panc02 cells were harvested by treatment with Trypsin/EDTA cocktail or by scraping. c Because trypsin reduced mesothelin detection, all subsequent experiments on adherent cells were performed using chelating agents without trypsin. Mesothelin expression was detectable on mouse-mesothelin-transfected HEK, and several mouse cancer cell lines: AB12 mesothelioma, TRAMP-C3 prostate <t>adenocarcinoma,</t> the JWF2 keratinocyte squamous cell carcinoma, and NIH/3 T3 cells
Human Ovarian Adenocarcinoma, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tov+21g/pmc04774190-28-3-1?v=ATCC
Average 93 stars, based on 1 article reviews
human ovarian adenocarcinoma - by Bioz Stars, 2026-07
93/100 stars
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90
BioResource International Inc type i ovca cell lines tov-21g
Expression of murine mesothelin. a RT-PCR detection of mesothelin (M, 449 base pairs) and control GAPDH (G, 473 bp) RNA in Panc02 cells. b Mesothelin protein surface expression – cells were stained with rat anti-mouse mesothelin monoclonal antibody B35 ( black line ) or normal rat IgG ( gray line ) and Cy5-conjugated donkey anti-rat IgG and analyzed by flow cytometry. Top right panels show that Panc02 surface detection is reduced by trypsin treatment compared to scraping of the adherent cells into solution. Adherent Panc02 cells were harvested by treatment with Trypsin/EDTA cocktail or by scraping. c Because trypsin reduced mesothelin detection, all subsequent experiments on adherent cells were performed using chelating agents without trypsin. Mesothelin expression was detectable on mouse-mesothelin-transfected HEK, and several mouse cancer cell lines: AB12 mesothelioma, TRAMP-C3 prostate <t>adenocarcinoma,</t> the JWF2 keratinocyte squamous cell carcinoma, and NIH/3 T3 cells
Type I Ovca Cell Lines Tov 21g, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tov+21g/pmc08870566-36-4-13?v=BioResource+International+Inc
Average 90 stars, based on 1 article reviews
type i ovca cell lines tov-21g - by Bioz Stars, 2026-07
90/100 stars
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90
China Center for Type Culture Collection tov-21g
Expression of murine mesothelin. a RT-PCR detection of mesothelin (M, 449 base pairs) and control GAPDH (G, 473 bp) RNA in Panc02 cells. b Mesothelin protein surface expression – cells were stained with rat anti-mouse mesothelin monoclonal antibody B35 ( black line ) or normal rat IgG ( gray line ) and Cy5-conjugated donkey anti-rat IgG and analyzed by flow cytometry. Top right panels show that Panc02 surface detection is reduced by trypsin treatment compared to scraping of the adherent cells into solution. Adherent Panc02 cells were harvested by treatment with Trypsin/EDTA cocktail or by scraping. c Because trypsin reduced mesothelin detection, all subsequent experiments on adherent cells were performed using chelating agents without trypsin. Mesothelin expression was detectable on mouse-mesothelin-transfected HEK, and several mouse cancer cell lines: AB12 mesothelioma, TRAMP-C3 prostate <t>adenocarcinoma,</t> the JWF2 keratinocyte squamous cell carcinoma, and NIH/3 T3 cells
Tov 21g, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tov+21g/pmc04317845-8-2-9?v=China+Center+for+Type+Culture+Collection
Average 90 stars, based on 1 article reviews
tov-21g - by Bioz Stars, 2026-07
90/100 stars
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N/A
Spontaneous immortalised epithelial ovarian cancer cell line derived from a patient never exposed to chemotherapy or radiation therapy. Cell line retains cahracteristics of the original epithelial ovarian cancers from which it was derived.
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Expression of murine mesothelin. a RT-PCR detection of mesothelin (M, 449 base pairs) and control GAPDH (G, 473 bp) RNA in Panc02 cells. b Mesothelin protein surface expression – cells were stained with rat anti-mouse mesothelin monoclonal antibody B35 ( black line ) or normal rat IgG ( gray line ) and Cy5-conjugated donkey anti-rat IgG and analyzed by flow cytometry. Top right panels show that Panc02 surface detection is reduced by trypsin treatment compared to scraping of the adherent cells into solution. Adherent Panc02 cells were harvested by treatment with Trypsin/EDTA cocktail or by scraping. c Because trypsin reduced mesothelin detection, all subsequent experiments on adherent cells were performed using chelating agents without trypsin. Mesothelin expression was detectable on mouse-mesothelin-transfected HEK, and several mouse cancer cell lines: AB12 mesothelioma, TRAMP-C3 prostate adenocarcinoma, the JWF2 keratinocyte squamous cell carcinoma, and NIH/3 T3 cells

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Murine mesothelin: characterization, expression, and inhibition of tumor growth in a murine model of pancreatic cancer

doi: 10.1186/s13046-016-0314-2

Figure Lengend Snippet: Expression of murine mesothelin. a RT-PCR detection of mesothelin (M, 449 base pairs) and control GAPDH (G, 473 bp) RNA in Panc02 cells. b Mesothelin protein surface expression – cells were stained with rat anti-mouse mesothelin monoclonal antibody B35 ( black line ) or normal rat IgG ( gray line ) and Cy5-conjugated donkey anti-rat IgG and analyzed by flow cytometry. Top right panels show that Panc02 surface detection is reduced by trypsin treatment compared to scraping of the adherent cells into solution. Adherent Panc02 cells were harvested by treatment with Trypsin/EDTA cocktail or by scraping. c Because trypsin reduced mesothelin detection, all subsequent experiments on adherent cells were performed using chelating agents without trypsin. Mesothelin expression was detectable on mouse-mesothelin-transfected HEK, and several mouse cancer cell lines: AB12 mesothelioma, TRAMP-C3 prostate adenocarcinoma, the JWF2 keratinocyte squamous cell carcinoma, and NIH/3 T3 cells

Article Snippet: OVCAR3 (ATCC HTB-161), human ovarian adenocarcinoma, was the gift of Dr. Anne Kellogg (East Carolina University).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Staining, Flow Cytometry, Transfection